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| MENDIBURO,MARÍA JOSE; FLORES,SEBASTIÁN; PIZARRO,FERNANDO; ARREDONDO,MIGUEL. |
| Heme oxygenase-1 is a microsomal enzyme that, when induced by stress, protects the cells from oxidative injury. Heme oxygenase-1 participates in the cleavage of the heme ring producing biliverdin, CO and ferrous Fe. The released Fe becomes part of intracellular Fe pool and can be stored in ferritin or released by an iron exporter. The mechanism by which heme enters cells is not completely understood, although it had been suggested that it might be internalized by an endocytosis process. In this study, we expressed a full-length Heme oxygenase-1 cDNA in Caco-2 cells and measured intracellular iron content, heme-iron uptake and transport and immunolocalization of heme oxygenase-1 in these cells. We found that heme oxygenase-1 expressing cells showed... |
| Tipo: Journal article |
Palavras-chave: Iron; Heme; Heme oxygenase; Caco-2 cells. |
| Ano: 2006 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000100023 |
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| ARREDONDO,MIGUEL; MARTÍNEZ,RONNY; NÚÑEZ,MARCO T; RUZ,MANUEL; OLIVARES,MANUEL. |
| Interactions of micronutrients can affect absorption and bioavailability of other nutrients by a number of mechanisms. In aqueous solutions, and at higher uptake levels, competition between elements with similar chemical characteristics and uptake process can take place. The consequences of these interactions may depend on the relative concentrations of the nutrients. In this work, we measure the effects of increasing concentrations of iron, zinc, and copper on iron and copper uptake in Caco-2 cells. Intracellular Fe or Cu levels were affected by incubating with increased concentrations of metals. However, when the cells already had different intracellular metal concentration, the uptake of Fe or Cu was nor affected. In competition studies, we showed that... |
| Tipo: Journal article |
Palavras-chave: Competition; Interaction; Metals; Uptake. |
| Ano: 2006 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000100011 |
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| NUÑEZ,MARCO T; GARATE,MARCO A; ARREDONDO,MIGUEL; TAPlA,VICTORIA; MUÑOZ,PATRICIA. |
| Cells tightly regulate iron levels through the activity of iron regulatory proteins (IRPs) that bind to RNA motifs called iron responsive elements (IREs). When cells become iron-depleted, IRPs bind to IREs present in the mRNAs of ferritin and the transferrin receptor, resulting in diminished translation of the ferritin mRNA and increased translation of the transferrin receptor mRNA. Similarly, body iron homeostasis is maintained through the control of intestinal iron absorption. Intestinal epithelia cells sense body iron through the basolateral endocytosis of plasma transferrin. Transterrin endocytosis results in enterocytes whose iron content will depend on the iron saturation of plasma transferrin. Cell iron levels, in turn, inversely correlate with... |
| Tipo: Journal article |
Palavras-chave: Metal ions; Intestinal absorption; Ferritin; Transferrin; Homeostasis. |
| Ano: 2000 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602000000200013 |
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